However, some of this advantage is lost if the feed is a mixture of BSA and Tg since, in this case, Tg binding leads to greater diffusional hindrance for BSA.Ĭore-shell resins Diffusional hindrance Dynamic binding capacity Flow-through purification Modeling.Ĭopyright © 2021. Column measurements show that, despite the higher static capacity of Capto Core 400 for BSA, the dynamic binding capacity is greater for Capto Core 700 as a result of its faster kinetics. The resin average bead size is 90.7 m with a range of 50-130 m, the shell thickness is 4.18 m with a range of 3-6 m and a standard deviation of 0.55 m, and the pore radius, obtained by inverse size exclusion chromatography, is 50.4 1.3 nm. Adsorbed Tg further hinders diffusion of BSA in both resins.
These values decrease dramatically for Tg to 0.022 × 10 -7 and 0.088 × 10 -7 cm 2/s and to 0.13 × 10 -7 and 0.59 × 10 -7 cm 2/s for Capto Core 400 and 700, respectively. For BSA, core and shell effective pore diffusivities are about 0.25 × 10 -7 and 0.6 × 10 -7 cm 2/s, respectively, for Capto Core 400, and about 1.6 × 10 -7 and 2.6 × 10 -7 cm 2/s, respectively, for Capto Core 700. HiTrap Capto Q, Capto S and Capto DEAE columns provide fast, reproducible and easy separations in a convenient format. Mass transfer in both resins is affected by diffusional resistances through the shell and within the adsorbing core. HiTrap Capto Q, HiTrap Capto S and HiTrap Capto DEAE are prepacked 1 mL and 5 mL columns for screening of selectivity, binding and elution conditions, as well as small scale purifications. However, for the much larger Tg, the attainable capacity is substantially larger for Capto Core 700. Effective pore diffusivities values in the core are 1.6 × 10-7 and 0.16 × 10-7 cm 2 /s for BSA and Tg, respectively. Because of the smaller pores and higher surface area, the BSA binding capacity of Capto Core 400 is approximately double that of Capto Core 700. Although shell thicknesses are comparable (3.6 and 4.2 µm for Capto Core 400 and 700, respectively), the two resins differ substantially in pore size (pore radii of 19 and 50 nm, respectively). Both resins are agarose-based and contain an adsorbing core surrounded by an inert shell. chromatography resins with different pore size and ligand chemistries. Nuvia Q Resin can significantly improve productivity while contributing to reduced capital costs, space requirements, and cycle time for downstream biotherapeutic purification.Structural and functional characteristics of the two core-shell resins Capto™ Core 400 and 700, which are useful for the flow-through purification of bioparticles such as viruses, viral vectors, and vaccines, are compared using bovine serum albumin (BSA) and thyroglobulin (Tg) as models for small and large protein contaminants. anion exchange resins, Nuvia aPrime and Capto Adhere ImpRes, compared with the. This exceptional binding capacity makes Nuvia Q the anion exchanger of choice for biopharmaceuticals as upstream processes deliver higher concentrations of feed titers.Īlong with high binding capacity, Nuvia Q Resin also delivers excellent performance for polishing applications.
Nuvia Q Resin delivers high binding capacity over a range of pH and flow rates, providing a wide experimental design space for process developers. Nuvia Q Resin is an ultra-high capacity, next-generation anion exchange resin.
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